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KMID : 0903520080510040258
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2008 Volume.51 No. 4 p.258 ~ p.262
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Determination of Biosynthetic Pathway of Decursin in Hairy Root Culture of Angelica gigas
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Ji Xiu-Hong
Huh Bum
Kim Soo-Un
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Abstract
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To establish the biosynthetic pathway of decursin in Angelica gigas Nakai, feeding experiment with stable isotope-labeled precursors were conducted. Umbelliferone and decursin were labeled with deuterium at C-3. The umbelliferone, the decursin, and other comercially available putative precursors, L-phenylalanine-ring-d5 and trans-cinnamic acid-d7, were fed to the hairy root culture of A. gigas. Each deuterated compound was incorporated into decursinol, decursinol angelate, and decursin as determined by mass spectrometric analysis. These findings confirmed the coumarin biosynthesis pathway sequence is composed of phenylalanine, cinnamic acid, umbelliferone, decursinol, and decursin.
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KEYWORD
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Angelica gigas, decursin, decursinol, deuterium-label, umbelliferone
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